These results suggest that chronic untreated diabetes impairs the permeability of the hippocampal blood–brain barrier by down-regulating occludin and claudin-5, indicating that chronic untreated diabetes may cause hippocampus-dependent dysfunction. In addition, ZDF rats showed ultrastructural changes with phagocytic findings in the blood vessels. Three layers of dentate gyrus: Molecular (M), Granule cell (G), and Hilus (H). Tight junction markers, such as occludin and claudin-5, were significantly decreased in the hippocampi of ZDF rats compared to those of ZLC rats. Light micrograph of a section through the rat hippocampus (Giemsa stain, scale bar500 µm). In addition, ZDF rats showed ultrastructural changes with phagocytic findings in the blood vessels. ZDF rats showed significantly increased sodium fluorescein leakage in the hippocampus. Tight junction markers, such as occludin and claudin-5, were significantly decreased in the hippocampi of ZDF rats compared to those of ZLC rats.
ZDF rats showed significantly increased sodium fluorescein leakage in the hippocampus. In addition, tight junction markers, such as zona occludens-1, occludin and claudin-5, were quantified by western blot analysis. We evaluated the integrity of the blood–brain barrier by measuring sodium fluorescein extravasation and blood vessel ultrastructure. 5.2 Hippocampus The term hippocampal formation typically refers to the dentate gyrus, the hippocampus proper (i.e., cornu ammonis), and the subicular cortex.
Forty-week-old diabetic (Zucker diabetic fatty, ZDF) rats and littermate control (Zucker lean control, ZLC) rats were used in this study. In the present study, we investigated the effects of type 2 diabetes-induced hyperglycemia on the integrity of the blood–brain barrier and tight junction markers in the rat hippocampus.
Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Koreaĭepartment of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung-Wonju National University, Gangneung 25457 South Koreaĭepartment of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, South Koreaĭepartment of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, South Korea KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul 08826, South Korea
0 kommentar(er)
